When it comes to identifying proteins in research or developing therapies for clinical use, antibodies are the most suitable option. Antibodies have become indispensable for molecular biology and can be produced in a wide variety of hosts. However, since then the original technique has been modified with variants that meet any special requirements.
With so many options available, why is it greatest to choose rabbit monoclonal antibodies across other monoclonal such as mice? To know about details of rabbit polyclonal antibody production visit www.bosterbio.com/services/custom-polyclonal-antibody-development-service.
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Why Choose Rabbit Monoclonal Antibodies?
Rabbit monoclonal antibodies are similar to mouse monoclonal antibodies in terms of consistency, but they have superior specificity and sensitivity. Rabbit spleen cells are vaccinated and united with partner cells to create an immortal cell line that produces antibodies.
The antibodies are derived from a single clone and characterized for performance in applications. The best clone is then selected for antibody production. Monoclonal antibodies have been traditionally bred in rodents such as mice because they are cheap and easier to use.
The use of rabbits as the host species is increasingly becoming the preferred monoclonal antibody choice for use in various researches, diagnostic, and clinical applications because the rabbit’s immune system is capable of producing more high-affinity antibodies in mice.
Rabbit monoclonal antibodies also respond better to immunogens and have higher epitope affinity and specificity. With antibodies bred in rodents, the protein is more likely to be recognized as an antigen on its own, making it less immunogenic.